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DNA Isolation

CM Cecilia Martini
RT Riccardo Torelli
TG Theun de Groot
EC Elena De Carolis
GM Grazia Angela Morandotti
GA Giulia De Angelis
BP Brunella Posteraro
JM Jacques F. Meis
MS Maurizio Sanguinetti
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Genomic DNA from C. parapsilosis isolates was obtained with material/equipment of Roche Diagnostics GmbH (Mannheim, Germany). Briefly, we suspended each isolate in 400-μL MagNA Pure Bacteria Lysis Buffer in a tube prefilled with ceramic beads (MagNA Lyser Green Beads), and we used the MagNA Lyser instrument for 30 s at 6,500 rpm to lyse fungal cells mechanically. DNA was then extracted and purified using the MagNA Pure LC instrument with the MagNA Pure DNA isolation kit III (bacteria, fungi), according to the recommendations of the manufacturer.

Copyright and License information:  ©2020 Martini, Torelli, de Groot, De Carolis, Morandotti, De Angelis, Posteraro, Meis and Sanguinetti.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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