Cardiac function

Tobias Borchert; Annika Hess; Mario Lukačević; Tobias L. Ross; Frank M. Bengel; James T. Thackeray

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

Cardiac function

TB Tobias Borchert

AH Annika Hess

ML Mario Lukačević

TR Tobias L. Ross

FB Frank M. Bengel

JT James T. Thackeray

This method is extracted from research article: Eur J Nucl Med Mol Imaging, Mar 2020

Angiotensin-converting enzyme inhibitor treatment early after myocardial infarction attenuates acute cardiac and neuroinflammation without effect on chronic neuroinflammation

DOI: 10.1007/s00259-020-04736-8

Ask a question

Favorite

To determine infarct size and assess contractile function, electrocardiographically gated perfusion SPECT scans were acquired as described previously [15]. Briefly, under isoflurane anesthesia, mice were injected with ^99mTc-sesatamibi (116 ± 13 MBq) as a bolus via a catheter inserted into a lateral tail vein. After 30 min of distribution, mice were positioned prone on the animal bed and ECG-gated listmode images were acquired using the Explore speCZT (TriFoil Imaging) equipped with a full ring of cadmium–zinc–telluride (CZT) detectors and a mouse 7-pinhole collimator. Images were acquired over ~ 25 min as described [12, 15], and reconstructed as a summed image and into 8 cardiac gates for functional analysis using an iterative algorithm. Additional details are provided in the online supplement.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol