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2.3. Quantification of Triglycerides
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Intracellular triglyceride levels were quantified using a colorimetric assay that is based on enzymatic hydrolysis of the triglycerides to produce glycerol and free fatty acids, following manufacturer’s instructions (Cayman Chemical, Ann Arbor, MI, USA). The triglyceride content was normalized to the protein content that was measured using the BCA protein assay kit (Pierce; Thermo Scientific, Waltham, MA, USA).
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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