4.8. Proteome Profile/Human Phospho-Kinase Array

For molecular pathway analysis we used a “human phospho-kinase array” ready-made kit which analyzes alterations of kinase signaling in response to exposure to selected three agents (BPA, OP, HBCD) which showed the most significant response at most of the previous tests. The array analyses 43 kinase phosphorylation sites and 2 related total proteins with carefully selected specific capture antibodies. The experimental technique and analyses were according to manufacturer’s booklet which also contains a list of the targets and phosphorylation sites (Proteome Profiler; R&D Systems, Minneapolis, MN, USA). This analysis was performed on the two-month exposed HME1 cells to low concentration of BPA, OP, HBCD and vehicle (DMSO). selected results were confirmed by Western blot.