Cell viability analysis

Cell viability was assessed using an MTT assay to measure the results of lung cancer cell proliferation. Cells were seeded in 96‐well plates with 3000 cells/well in at least three replicates. Then, 10 hours later, the confluent monolayers were formed, and the medium was changed to medium containing aspirin for another 24, 36 or 72 hours. We then supplemented 10 μL MTT (5 mg/mL) into each well, and after four hours incubation the medium was discarded and MTT in 150 μL DMSO was added into each well. At OD_490nm, the absorbance values were measured via an absorbance reader.