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Evaluation of Pro-inflammatory Cytokines in LPS-Induced Murine RAW264.7 Macrophages
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After the infection of LPS (1µg/ml) to RAW 264.7 cells (5x105/well), in the presence or absence of TP5 and hybrid DEFB-TP5 peptide (30 to 60µg/ml). The expression level of a proinflammatory cytokine TNF-α, IL-1β and IL-6 were accessed by using ELISA kit (could-clone corp, Houston, USA). The levels were quantified at 450 nm absorbance.
Copyright and License information: ©2020 Ahmad, Li, Hanif, Hu, Wei, Zhang, Khan, Aihemaiti, Gulzar, Shahid, Si and Zhang
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