Determination of itraconazole solubility

The saturation solubility study method was employed for the determination of ITRA solubility.¹⁶ Double distilled water was used to prepare solvent mixtures. Binary compositions of triacetin: water were used from 0% to 100% by the mass fraction of triacetin. Then 10 g of binary solvents blends were taken in screw cap vials and were saturated by the addition of excess of drug. These vials were mounted in an orbital shaker (Remi, India) at 298.15 K and 100 rpm for 72 h. The saturation time of 72 h was established through the preliminary studies. After 72 h, these vials were removed and the solutions were filtered carefully with the help of micro filters of 0.45 µm. The filtrate was collected, diluted suitably, and was subjected to spectrophotometric analysis using a double beam ultraviolet spectrophotometer (Shimadzu, Japan) at 255 nm. All the experiments were performed in triplicate. The densities of pure solvent blends and filtered saturated blends were determined and used for the estimation of saturated solubilities in terms of mole fraction.