Statistical analysis

Independent continuous data were analyzed using one-way analysis of variance (ANOVA). When the ANOVA reached significance, individual t tests were performed between groups [21]. Repeated measures data were analyzed using a random intercept mixed model with a categorical effect of time, a categorical effect of group, and the group by time interactions. Data are presented as means ± standard error of the mean (SEM), unless otherwise indicated. A P value of 0.05 was considered significant. All statistical tests were performed using Stata v15 (StataCorp, College Station, TX), and graphics were created using Prism 8 (GraphPad, San Diego, CA).