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DAPI staining for detection of apoptosis
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The cells were grown on coverslips in 24 well plates and incubated with respective drug or inhibitor for 24 h. They were then washed with PBS, fixed with chilled methanol-EDTA for 10 min and rehydrated with PBS for 10 min at room temperature. Successively, the cells were treated with DAPI (0.5 μg/ml) for 2 min in the dark. The coverslips were then mounted on fluoromount G and viewed at 40X in the UV region using a fluorescence microscope (Olympus IX71).
Copyright and License information: Thamkachy et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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