Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.5. Nissl Body and Neuron Distribution in the Striatum

MT Mahnaz Taherianfard
MR Moslem Riyahi
MR Mostafa Razavi
ZB Zahedeh Bavandi
NR Narges Eskandari Roozbahani
MN Mohammad Mehdi Namavari
request Request a Protocol
ask Ask a question
Favorite

At the end of the experiments, the rats were deeply anesthetized and sacrificed. For Nissl-body and neuron distribution measuring, after brain tissue fixation by 10% formalin, automatic tissue processor prepared paraffin blocks; then, 5 μ coronal section of the brain blocks in the region of striatum neurons was prepared. In all sections, Nissl-body counting in six cells and cell counting in six parts of each slide section of the striatum in all of the groups by Cresyl violet and hematoxylin eosin staining were done.

Copyright and License information:  ©2020 Mahnaz Taherianfard et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A