Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.7. Nrf2 signaling using a reporter gene vector
Request a Protocol
Ask a
question
Favorite
HepG2 cells were transiently transfected with Antioxidant Response Elements (ARE)-luciferase plasmid (Dr J. Alam, Department of Molecular Genetics, Ochsner Clinic Foundation, USA). Cells were lysed and assayed for luciferase activity by Dual-Luciferase® Reporter (DLR™) Assay System (Promega). pTK-Renilla was used as an internal control vector (Promega).
Copyright and License information: ©2020 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
