Statistical significance for longitudinal analysis of serum antibodies was determined with a repeated-measures analysis of variance (ANOVA) followed by a Dunnett multiple comparisons test against phosphate-buffered saline (PBS) control. For analysis of multiple groups at 1 time point, appropriate 2-way ANOVA and 1-way ANOVA with a Dunnett multiple comparisons test was used. Significance was set at P ≤ .05, where *P ≤ .05, **P ≤ .01, ***P ≤ .001, and ****P ≤ .0001.
Additional details are given in supplemental Methods.
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