Materials

MJ Manasa Dogganal Jayappa
CR Chandrashekar Konambi Ramaiah
MK Masineni Allapuramaiah Pavan Kumar
DS Doddavenkatanna Suresh
AP Ashwini Prabhu
RD Rekha Punchappady Devasya
SS Sana Sheikh
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Healthy leaf and stem parts of M. frondosa were collected from natural forests of Dakshina Kannada (12.8158° N and 74.9241° E), Karnataka, India. The plant was authenticated by Flora of Madras Presidency (Gamble 1958) and a voucher specimen (No: MU/AB/DJM-02) was deposited in the Dept. of Applied Botany, Mangalore University. 1, 1-dipheny l-2-picrylhydrazyl (DPPH), α-amylase, starch and DNS were procured from Sigma-Aldrich. Methanol, zinc nitrate hexahydrate [Zn (NO3)2·6H2O] and methylene blue dye were procured from S.D. Fine chemicals. Murashige and Skoog medium (MS Media) and growth regulators were purchased from Hi-media (India). α-Glucosidase and p-nitrophenyl-beta-d-glucoside (P-PNPG) were purchased from SRL.

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