Nude Mouse Xenograft Model of NSCLC

A total of 40 BALB/C nude mice (4 weeks old, weighing 18–22 g) were purchased from the Experimental Animal Center of Inner Mongolia Medical University. Under sterile conditions, 0.2 mL of cell suspension (l × 10⁷ cells/mL) was pipetted with a 1 mL disposable syringe and slowly injected into the right armpit of the nude mouse. The long diameter of the tumor block >5 mm and felted lumps was indicative of the successful establishment of the NSCLC mouse model. After successful modeling, 0 μg/kg, 10 μg/kg, 20 μg/kg and 40 μg/kg of gallic acid were injected subcutaneously into the back of the neck of nude mice for 14 d (1 time/d). After administration, each mouse was intraperitoneally injected with 0.5% heparin (0.5 mL) for heparinization and then ocular blood samples were collected. The concentration of alanine aminotransferase (ALT), gamma-glutamyltransferase (γ-GT), blood urea nitrogen (BUN) and creatinine (Cr) were then detected after centrifugation. During the administration, the long diameter and short diameter of the transplanted tumors were measured by vernier calipers every 2 d, which was repeated in triplicate to obtain the average values. The tumor volume was then calculated: V = long diameter × short diameter²/2 and the growth curve of the transplanted tumors were plotted based on the values obtained. After 14 d, the nude mice were weighed and euthanized with carbon dioxide asphyxiation and the tumor was removed and weighted.