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Ectopic Over-Expression of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) Protein

LC Lun Cai
LG Leidy D. Caraballo Galva
YP Yibing Peng
XL Xiaobing Luo
WZ Wei Zhu
YY Yihong Yao
YJ Yun Ji
YH Yukai He
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MCF7 cells were transduced by ENPP1 gene (Addgene, Watertown, MA, United States) by utilizing Lipofectamine 2000 (Invitrogen, Carlsbad, CA, United States). The expression of ENPP1 was detected by Western blot (WB) analysis (Figure 5A).

Tumor cells overexpressing ENPP1 do not activate TCRTs. (A) Western Blot analysis showed the ectopic overexpression of ENPP1 in MCF7 cells after transient gene transfer. (B) LDH assay showed that TCR-Ts did not kill the MCF7 cells that overexpress ENPP1 protein. (C) ELISA assay indicated that overexpression of ENPP1 in MCF7 did not activate TCR-Ts to produce IFNγ.

Copyright and License information:  ©2020 Cai, Caraballo Galva, Peng, Luo, Zhu, Yao, Ji and He.
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