Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

4.5. p38 MAPK Assay

TS Tsuyoshi Shimo
HT Hiroaki Takebe
TO Tatsuo Okui
YK Yuki Kunisada
SI Soichiro Ibaragi
KO Kyoichi Obata
NK Naito Kurio
KS Karnoon Shamsoon
SF Saki Fujii
AH Akihiro Hosoya
KI Kazuharu Irie
AS Akira Sasaki
MI Masahiro Iwamoto
request Request a Protocol
ask Ask a question
Favorite

Cell extracts were incubated overnight with immobilized p38 MAPK (Thr180/Tyr182) monoclonal antibody (mAb) (#9219 Cell Signaling Technology). A kinase reaction was performed in the presence of 100 µM of cold ATP (Cell Signaling Technology) and 2 µg of ATF2 fusion protein (#9224S Cell Signaling Technology). The phosphorylation of ATF2 at Thr71 was measured by western blotting using phospho-ATF2 (Thr71) antibody (#9221S Cell Signaling Technology).

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A