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Mitochondrial (Mt) DNA quantification
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Total DNA was extracted from GC2 cells or FACS-isolated spermatocytes using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturer's instructions. mtDNA levels were assessed by qRT-PCR using primers specific to mitochondrial gene 16S, with nuclear apoB serving as a reference. All the primer sequences are provided in Supplementary Table S1.
Copyright and License information: ©2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
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