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In a separate set of Scn1a‐/+ mice and injected Scn1a fl/fl mice, the threshold for hyperthermia‐induced seizures was tested as described previously, 12 adapted for freely behaving animals as follows: following implantation of electrodes, a thermistor (MEAS‐G22K7MCD419, Measurement Specialties) was placed in the peritoneal cavity. In weeks 4‐5 after surgery (ie, P43‐49), a heat lamp was positioned above the mouse during video‐EEG recording. Core temperature was gradually increased (0.5°C every 2 minutes) until a seizure occurred, or 42.0°C was reached.
Copyright and License information: ©2020 The Authors. published by Wiley Periodicals, Inc. on behalf of International League Against Epilepsy
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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