At day 2 after exposure to PO stress, rats were tested for acoustic startle reactivity. Rats were placed in a Plexiglas tube attached to an accelerometer inside a dark, soundproof chamber (SR-Lab, San Diego Instruments, CA) and allowed to acclimate for 5 min (75-dB background noise) before the test session [35]. This background white noise was present throughout the session. The chamber and Plexiglas tube were cleaned with Quatricide between each animal. Before testing, an S-R calibrator tube was used to calibrate the chambers. The test session consisted of 30 trials with startle stimuli of three different decibel levels: a 750-ms burst of 95 dB, 105 dB, or 115 dB white noise was randomly presented 10 times each, separated by a 30-s fixed inter-trial interval. The maximum startle response (Vmax, arbitrary units) was recorded during the first 100 ms of each trial.
Copyright and License information: The Author(s) ©2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.