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Sequencing Reads Quality Control and Visualization
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We used FastQC v0.11.61 and Multiqc v.1.0 (Ewels et al., 2016) to perform quality control of raw sequencing data. Adapter trimming was performed by Trimmomatic v.0.36 (Bolger et al., 2014) with TruSeq3 and Nextera adapters (for RNA-Seq and ATAC-Seq, respectively) using 2:30:10 parameters and the minimum read length of 30 bp. To visualize genomic/transcriptomic alignments and coverages, we used the Integrative Genomic Viewer v.2.3.97 (IGV) (Robinson et al., 2011).
Copyright and License information: ©2020 Hovhannisyan, Saus, Ksiezopolska, Hinks Roberts, Louis and Gabaldón.
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