Biocompatibility assessment

Papacarie Duo and Brix 3000 were organized into two groups for the in vitro cytotoxicity and genotoxicity tests with human pulp fibroblasts (FP6) provided by the cell bank of the Laboratory of Cell Biology of the São Paulo State University (UNESP), Campus São José dos Campos, SP, Brazil. The entire research method was performed according to the guidelines of the International Organization of Standardization (ISO) 10993-5:2009 in duplicate.

The indirect contact test for the sensitization of fibroblastic cells was performed with extracts of both products tested, produced from the diffusion of the components from each product to the culture medium, and put in contact with the fibroblastic cells. Therefore, 0.2 grams of each material was placed at the bottom of the 24-well plate (Prolab, São Paulo, SP, Brazil) and covered with 1 ml of DMEM (Dulbecco’s Modified Eagle Medium – LGC Biotecnologia, Cotia, SP, Brazil). The plates were incubated in the dark for 24 h at 37 °C to form the original extracts (1:1) of each material, which were then serially diluted (1:2, 1:4, 1:8, 1:16, and 1:32) prior to testing.