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Measurement of cGMP levels and PKG activity.

CR Christian Riehle
EW Eric T. Weatherford
AW Adam R. Wende
BJ Bharat P. Jaishy
AS Alec W. Seei
NM Nicholas S. McCarty
MR Monika Rech
QS Qian Shi
GR Gopireddy R. Reddy
WK William J. Kutschke
KO Karen Oliveira
KP Karla Maria Pires
JA Joshua C. Anderson
ND Nikolaos A. Diakos
RW Robert M. Weiss
MW Morris F. White
SD Stavros G. Drakos
YX Yang K. Xiang
EA E. Dale Abel
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cGMP levels in whole heart tissue were determined using a cGMP enzyme immunoassay kit (Cayman Chemical) according to the manufacturer’s instruction and normalized to tissue weight. PKG activity in whole heart tissue was determined by colorimetric analysis using a PKG assay kit (CycLex) according to the manufacturer’s instruction.

Copyright and License information:  ©2020 American Society for Clinical Investigation

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