2.6. Acetic acid-induced writhing test

QL Qianjin Liu
LS Ling-Yan Su
CS Chunli Sun
LJ Lijin Jiao
YM Ying Miao
MX Min Xu
RL Rongcan Luo
XZ Xin Zuo
RZ Rongbin Zhou
PZ Ping Zheng
WX Wei Xiong
TX Tian Xue
YY Yong-Gang Yao
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Pain sensitivity was evaluated by measuring the acetic acid-induced writhing responses as previously described [31], as acetic acid could induce abdominal contractions and hind limb stretching. Mice were placed into open polyvinyl cages (20 × 40 × 15 cm) immediately after acid challenge, and abdominal constrictions were counted cumulatively over a period of 30 min.

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