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Formalin-fixed paraffin-embedded tissue samples were acquired from USB Biomax, Inc., the Yale Department of Pathology, or the Juvenile Diabetes Research Foundation Network for Pancreatic Organ Donors with Diabetes. Tissue sections were stained by direct or indirect immunofluorescence with an anti–dopamine D2 or D3 receptor antibody and costained with antibodies for insulin (β-cells), glucagon (α-cells), somatostatin (γ-cells), and polypeptide Y (PP cells), as described in Supplemental Table 1 (supplemental materials are available at http://jnm.snmjournals.org).
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