Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Rats were placed individually on a hot plate analgesia meter (Columbus instruments, USA) maintained at a constant temperature of 55 ± 0.1 °C after observing them for 5 min in the cage [24]. The paw withdrawal latency (PWL) was recorded as the time taken to exhibit distinct pain behaviour either by hindpaw licking or hindpaw flicks (whichever occurred first). Rats that did not respond within 30 s were removed from the hot plate to prevent tissue damage [25].
Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
