Cell culture

DK Dong Hoon Kang
DL Doo Jae Lee
SL Sunmi Lee
SL So-Young Lee
YJ Yukyung Jun
YK Yerin Kim
YK Youngeun Kim
JL Ju-Seog Lee
DL Dae-Kee Lee
SL Sanghyuk Lee
EJ Eek-Hoon Jho
DY Dae-Yeul Yu
SK Sang Won Kang
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All CRC and HEK293 cells were purchased from American Type Culture Collection (Manassas, VA, USA). SW480, DLD1, CoLo205, Colo741, and SW620 cells were cultured and passaged in RPMI 1840 Medium supplemented with 10% fetal bovine serum. HEK293 and RKO cells were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. HT29 cells were cultured in McCoy’s 5A medium supplemented with 10% fetal bovine serum. Mycoplasma contamination was periodically tested in the cell culture supernatants using mycoplasma detection kit (Biotool, USA).

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