4.5. miRNA Mimic or Inhibitor Transfection

Transfection was performed using the GenMute siRNA Transfection Reagent (SignaGen Laboratories, Rockville, MD, US) according to the manufacturer’s instructions. The negative control as well as miR-296 mimic and inhibitor were purchased from Dharmacon (Lafayette, CO, USA). The transfection buffer was diluted, incubated to a final concentration of 10 nM of miR-296 mimic or inhibitor for 15 m at room temperature, and then added to each well. We seeded 2 × 10⁴ cells on 6-well plates and incubated them at 37 °C with 5% CO₂ for 24 h. The cells were incubated in fresh culture medium 30 min before the transfection. After 24 h, the cells were transfected with a miR-296 mimic or inhibitor and the negative control according to the manufacturer’s protocol.