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Extraction of Glycolipids

RH Rebecca Hollenbach
BB Benjamin Bindereif
US Ulrike S. van der Schaaf
KO Katrin Ochsenreither
CS Christoph Syldatk
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For glycolipid extraction, 3.42 mL of extraction solvent and 1.1 mL of double distilled water were mixed with the reaction tube content (2.5 g) in a rotator with vortex mixer (program U2) at an agitation rate of 90 rpm. Temperature and time were set to 50°C and 20 min, respectively, after which the upper organic phase was collected for HPLC analysis.

Three different extraction solvents were tested under similar conditions as stated above, but the volume was halved (1.71 mL of extraction solvent and 0.55 mL of double distilled water per mL of DES): ethyl acetate (EtAc), dimethyl carbonate (DMC) and chloroform were tested as extractants. To evaluate the extraction performances, 5 mg of glucose decanoate was incubated in 1 mL of both DES for at least 5 h and afterward extracted and analyzed by HPLC-ELSD. The extraction efficiency was calculated as follows:

Copyright and License information:  ©2020 Hollenbach, Bindereif, van der Schaaf, Ochsenreither and Syldatk.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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