NMR General Experimental Procedure

NMR spectra (¹H-NMR, ¹H-¹H COSY, ¹H-¹³C HSQC, and ¹H-¹³C HMBC) were measured on an Avance III HD 500 NMR spectrometer, operating at 500.13 MHz for ¹H and 125.75 MHz for ¹³C, and an Avance III HD 700 NMR spectrometer, operating at 700.13 MHz for ¹H and 175.75 MHz for ¹³C (Bruker BioSpin). Both spectrometers were equipped with a TCI CryoProbe (5 mm for Avance III HD 500 and 1.7 mm for Avance III HD 700). Spectrometer control and data processing was accomplished using the software Topspin ver. 3.6.1 (Bruker BioSpin). All NMR measurements were recorded at 25°C in D₂O, and chemical shifts were left uncorrected.