Burst Size Analysis

1 mL of exponential-growth-phase culture of Xcc in NB (10⁸ CFU/mL) and phage suspension were mixed at MOI of 0.1. The mixture was incubated at 24°C for 5 min to allow phage adsorption. Immediately after, the mixture was diluted to 10^–4 in 50 mL Erlenmeyer flasks. Samples were taken from the diluted fraction at ten-min intervals, serially diluted ten-fold and spotted on NA plates using the agar overlay technique. The experiment was repeated three times. The latent period was expressed as the time interval between phage adsorption (which does not include the 5-min pre-incubation time) and the first burst (Drulis-Kawa et al., 2011; Rigvava et al., 2013). Burst size was calculated as the ratio between the final count of liberated phage particles and the initial count time of infected bacterial cells during the latent period.