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Enzyme linked immunosorbent assay (ELISA)
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After treatments, culture supernatants from THP-1 macrophages were collected and soluble IL-18, IL-1β, and 4-HNE levels were measured by ELISA kits (Elabscience Biotechnology Co. Ltd., Wuhan, China) following the manufacturer’s protocols.
Copyright and License information: ©2020 Cong et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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