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padi2-201a cloned into pCS2+8 (described above) was linearized using NotI restriction digest, and RNA was in vitro transcribed using the mMessage mMachine Sp6 kit (Ambion). RNA was cleaned up using an RNeasy Minikit column (Qiagen) and injected into single cell embryos (3 nl of 100 ng/μl). Embryo lysates were collected as described for Western blotting at 2 dpf and 5 dpf.
Copyright and License information: ©2020 Golenberg et al.
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