Acute pancreatitis was induced based on established protocols. Caerulein (MedChemExpress) was reconstituted in sterile PBS. Adult mice (6 to 10 wk old) were injected (individual dose: 75 μg/kg), given as eight hourly i.p. injections on 2 consecutive days during the light cycle. Mice were s.c. administered sustained release buprenorphine as an analgesic prior to the first caerulein injection.
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