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The exponentially grown NPC cells were cultured in 96‐well plate for 12 hr. Within 24 hr, siRNA was added to interfere with DLX6‐AS1 expression, and siRNA transfection was carried out with Lipofectamine RNAimax reagent (Invitrogen). The medium was replaced at a specified time with a complete medium encompassing 10 μl MTT reagent, and then the cells were incubated at 37°C for 1 hr. Vmax microspectrophotometer (Molecular Devices) was placed to measure absorbance at 570 nm.
Copyright and License information: ©2019 Chinese Society of Clinical Oncology. published by Wiley Periodicals, Inc.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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