2.4. Induction of Colitis

Colitis was induced by the administration in ICR mice with drinking water dissolving 4% (w/v) DSS for 9 days (ad libitum) (Figure 1). For each experiment, the mice were administrated orally with different material once a day for 9 days; (1) Control group: mice drank normal water and were fed vehicle (0.9% saline); (2) DSS group: mice drank DSS-containing water and were fed vehicle; (3) 5-aminosalicylic acid (5-ASA) group (positive control): mice drank DSS-containing water and were administrated 75 mg/kg/day of 5-ASA; (4) CEE 100 mpk group: mice drank DSS-containing water and were administrated 100 mg/kg/day of CEE; (5) CEE 200 mpk group: mice drank DSS-containing water and were administrated 200 mg/kg/day of CEE. All tested materials were dissolved in 0.9% saline, and the administration of each materials was started at the same time as the DSS treatment.

Design of animal experiments.