Peritoneal Lavage

Peritoneal cells were collected as previously described (Larson and Mitre 2012). Briefly, 5 ml of 1× HBSS was injected into the peritoneal cavity of mice and the abdomen was manually agitated for at least 1 min. Cells were removed from the peritoneal cavity by collecting lavage fluid using a transfer pipet. Lavage fluid was measured and leukocytes were counted using a hemocytometer. Total cell counts from each mouse were corrected to account for any HBSS not recovered. Numbers of each cell type were determined by multiplying the total corrected number of peritoneal cells by the cell type percentages that were determined by flow cytometry. No peritoneal lavages were performed on mice in the long-term, tumor development study.