Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Cells were treated with nanoparticles (50 nM anti-miR, 50 nM siRNA) for 48 h. Cells were stained with 2 μg/mL of DAPI (4′, 6-diamidino-2′-phenylindole dihydrochloride) and observed under EVOS xl fluorescence microscope (Thermo, US). Apoptotic nuclei (condensed and fragmented morphology) were counted and presented as a percentage of total nuclei per observation field.
Copyright and License information: ©2020
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
