Cell lines.

The human NCI-H522 non–small cell lung cancer adenocarcinoma cell line originated from an NCI-60 cell line panel obtained from Daniel Haber at Massachusetts General Hospital. The HEK-293T human embryonic kidney cell line originated from Richard Van Etten’s laboratory at Tufts Molecular Oncology Research Institute. The human Jurkat T cell line has been maintained in the Tsichlis lab since his work at Fox Chase University and originated from David Wiest. NCI-H522, HEK-293T, and Jurkat cells were grown in Dulbecco’s modified Eagle’s medium (MilliporeSigma, catalog no. D5796) and RPMI-1640 (MilliporeSigma, catalog no. R8758), respectively. The media were supplemented with penicillin/streptomycin, nonessential amino acids, L-glutamine, and 10% FBS. Cells were used within 3 months of resuscitation. Cell lines were periodically checked for mycoplasma.