C. elegans motility assays and analysis

L4 stage worms were picked from mixed population plates and transferred to clean NGM growth plates for incubation at 15, 20 or 25°C for 24 h. Subsequently, worms were moved to the assay room and allowed to acclimate for 20 min. One strain at a time, worms were washed from NGM plates to food-free video plates with 2 ml of M9, allowed to acclimate to M9 buffer for 15 s prior to a 1-min video recording. Videos were collected using the WormLab platform (MBF Bioscience, VT). After videos were taken, worm movement behavior was analyzed using the WormTracker software (MBF BioScience, version 2019.1.2). Body bends from the mid-point body location of each worm tracked were counted. The total number of body bends was divided by the track length(s) to give the frequency of body bends per second. This was compared across strains within each temperature using the Kruskal–Wallis ANOVA with post hoc Dunn’s test (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).