D. Tissue Microarray Construction

MP Mirkka Pennanen
OT Olli Tynninen
SK Soili Kytölä
PE Pekka Ellonen
HM Harri Mustonen
IH Ilkka Heiskanen
CH Caj Haglund
JA Johanna Arola
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Tissue microarray (TMA) blocks were constructed from archived surgical formalin-fixed, paraffin-embedded specimens. Representative areas of each tumor were chosen from H&E-stained slides. Using a semiautomatic TMA instrument (Beecher Instruments), three 1-mm cores were obtained from each histologically benign (Weiss score 0-2) tumor, and 6 cores were taken from the histologically malignant (Weiss score 3-9) tumors. In addition, 2 cores from the normal adrenal cortex were taken from each specimen.

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