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Immunization and in vitro stimulation

AM Asaf Madi
AP Asaf Poran
ES Eric Shifrut
SR Shlomit Reich-Zeliger
EG Erez Greenstein
IZ Irena Zaretsky
TA Tomer Arnon
FL Francois Van Laethem
AS Alfred Singer
JL Jinghua Lu
PS Peter D Sun
IC Irun R Cohen
NF Nir Friedman
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Mice were injected intra-peritonealy (IP) with 100 μg of either Chicken Ovalbumin (OVA) or peptide 277 (p277) emulsified in CFA (1:1 ratio). Spleens were harvested on day 7 post immunization and T cells were extracted for TCR analysis. in vitro stimulation: T cells from spleens of immunized mice were harvested on day 7 and were re-stimulated with irradiated splenocytes and the relevant peptide antigen. Five of the OVA-immunized mice received a boost IP injection of 100 μg OVA + CFA on day 14, and spleens were harvested on day 60 for TCR analysis (Supplementary file 3).

Copyright and License information:  ©2017, Madi et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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