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To measure the activity of the promoter, we used a dual-luciferase reporter system. We inserted the promoter sequence of pri-miR-639 into the pGL3/luciferase-basic vector to construct pGL3/luciferase-pri-639-p715 (pGL3-miR-639). The luciferase activity was measured according to the protocol of the Dual-Luciferase Reporter Assay System (Promega, MI, USA).
Copyright and License information: ©2019 The American Society of Gene and Cell Therapy.
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