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Mitochondrial membrane potential assay.

KI Kali R. Iyer
LW Luke Whitesell
JJ John A. Porco, Jr.
TH Thomas Henkel
LB Lauren E. Brown
NR Nicole Robbins
LC Leah E. Cowen
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Loss of mitochondrial membrane potential over time was visualized using MitoTracker Red (Molecular Probes) or the reversible cationic dye tetramethylrhodamine ethyl ester (TMRE) (Molecular Probes). Details are provided in Text S1. Cells were imaged by DIC microscopy and by the use of the DsRed (MitoTracker Red) or TexasRed (TMRE) channel on a Zeiss Axio Imager.MI microscope, with the exposure time remaining constant between samples. For the MitoTracker Red-treated cells, brightness was also quantified on a CytoFlex flow cytometer (Beckman).

Copyright and License information:  ©2020 Iyer et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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