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Cells were inoculated in 6-well plates. After cells reached confluency, they were scratched with a 10-µl disposable pipette tip. Migration of wound edges was measured at five random points on images acquired using an ECLIPSE TS100 microscope (Nikon), and the cell migration distance after 10 h was compared with the distance at 0 h. Data analysis was performed according to the previous protocol.20
Copyright and License information: The Author(s), under exclusive licence to Cancer Research UK ©2020
Note This work is published under the standard license to publish agreement. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution 4.0 International (CC BY 4.0).
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