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Cell viability assay

YL Yingge Liu
MZ Meiying Zhang
TH Tao He
WY Weili Yang
LW Lidong Wang
LZ Lirong Zhang
MG Mingzhou Guo
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Cells were plated into 96-well plates at a density of 2.5 × 103 cells per well, and cell viability was measured by the methyl thiazolyl tetrazolium (MTT) assay (KeyGEN Biotech, Nanjing, China) at 0, 24, 48, and 72 h. Absorbance was measured on a microplate reader (Thermo Multiskan MK3 [Thermo Fisher Scientific, Danvers, MA, USA]) at a wavelength of 490 nm.

Copyright and License information: The Author(s). ©2020
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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