4.7. Peroxisome Proliferator-Activated Receptor (PPAR)γ Reporter Gene Assay

CV-1 cells were transiently transfected with a plasmid mixture containing a PPARγ expression vector and the tk-PPRE-luciferase (Luc) vector, and then treated with test sample for 24 h. Luciferase activity in cell lysates was measured using the luciferase assay system (Promega). Data are reported as relative luciferase activity divided by β-galactosidase activity. All constructs were kindly gifted by Ronald M. Evans (The Salk Institute, La Jolla, CA, USA).