4.5. Cell Migration Assay

First, MDA-MB-231 cells were seeded in 12-well plates, at a density of 3 × 10⁵ cells per well, and allowed to settle overnight. Then, the cells attached to the bottom of the well were scratched to make a wound scratch perpendicular to the marker line. Next, the floating cells were washed off. DMEM was supplemented with various concentrations (0, 2.5, 5, 10 µM) of TSC-3C. The wound scratch was observed exactly at 0, 6, 12, and 24 h. The wound width was measured at different times, and the inhibitory effect of TSC-3C on MDA-MB-231 cell migration was analyzed.