Elevated Plus-Maze Test

The elevated plus-maze (EPM) test is a behavioral test used to analyze anxiolytic effects and general locomotor activity in laboratory animals. The maze is an elevated plus-shaped apparatus with two open and two enclosed arms. The total number of closed arms entries (frequency) and distance traveled by mice during the EPM test are quantitatively assessed as an indicator of general activity to evaluate the possibility of false-positive data in the FST.^32,33 In this study, a mouse was placed on the maze for a 5 min activity test. The test was recorded using EthoVision Basic V 3.1 program for automated analysis.