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The frozen tissue were previously lyophilized for 48 hrs and then thoroughly homogenized in distilled water. The samples were gently mixed with 12 M hydrochloric acid and hydrolysed by autoclaving at 120°C for 40 min. in O'‐ring screw‐capped Nalgene high‐temperature polypropylene tubes of 2‐ml capacity. Chloramine T reagent was added to the hydrolyzate, and the oxidation was allowed to proceed for 25 min. at room temperature. Finally, Ehrlich's aldehyde reagent was added to the samples and incubated at 65°C for 20 min., and the absorbance was read at 550 nm.
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