4.2. Cell cultures and Glucose Starvation

Ran Marciano; Barak Rotblat

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4.2. Cell cultures and Glucose Starvation

RM Ran Marciano

BR Barak Rotblat

This method is extracted from research article: Int J Mol Sci, Feb 2020

The Amuvatinib Derivative, N-(2H-1,3-Benzodioxol-5-yl)-4-{thieno[3,2-d]pyrimidin-4-yl}piperazine-1-carboxamide, Inhibits Mitochondria and Kills Tumor Cells under Glucose Starvation

DOI: 10.3390/ijms21031041

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All cell lines were obtained from the ATCC and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Biological Industries, Rechovot, Israel) containing Antibiotic-Antimycotic (Tivan Biotech, Rechovot, Israel) 1 mM sodium pyruvate (Biological Industries, Rechovot, Israel), and 10% fetal bovine serum (FBS) (Biological Industries, Rechovot, Israel). Cells were maintained at 37 °C and 5% CO₂. Glucose starvation: medium was changed to glucose-free DMEM containing Antibiotic-Antimitotic and 10% FBS. All compounds in the screen were diluted in DMSO.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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